Trisodium citrate Stock Solution

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Stock Solution Preparation Guide – Trisodium Citrate (C₆H₅O₇Na₃) Solution

Trisodium citrate (also called sodium citrate) is a white crystalline salt that dissolves readily in water. It serves as a buffering agent, chelating agent, and pH regulator in biochemical applications. A 1 M stock solution is conveniently prepared by dissolving the appropriate mass of trisodium citrate dihydrate in water and adjusting to the desired final volume.

Objective

Preparation of a 1 M trisodium citrate stock solution.

Composition

• Trisodium citrate (C₆H₅O₇Na₃) aqueous solution of defined molarity

Required Materials

• Trisodium citrate or trisodium citrate dihydrate solid
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Determine the amount of solid required by molarity and hydration state. For a **1 M solution** using **trisodium citrate dihydrate (MW ≈ 294.1 g/mol)**: weigh **294.1 g** and scale proportionally for other volumes (e.g., 29.41 g for 100 mL).
• Step 2
  Add the weighed solid into a beaker containing approximately 80 % of the final desired volume of deionized water. Stir until completely dissolved.
• Step 3
  Transfer the dissolved solution into a volumetric flask. Rinse the beaker with deionized water to ensure all solute is transferred. Add water to reach the final volume. Mix thoroughly.
• Step 4 (Optional)
  If sterile solution is required, filter through a **0.22 µm membrane** filter into a sterile container.

Note: Trisodium citrate solutions are typically near neutral to slightly alkaline. Label the stock solution container with concentration and preparation date. Store in a sealed container at room temperature or per your lab’s protocol.

Available Reagents