Sodium fluoride Stock Solution

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Stock Solution Preparation Guide – Sodium Fluoride (NaF)

Sodium fluoride (NaF) is an inorganic salt composed of sodium and fluoride ions. It appears as a white, odorless crystalline powder that dissolves readily in water to form clear solutions. In research and laboratory settings, aqueous solutions of NaF are commonly used as enzyme inhibitors and analytical reagents.

Objective

Preparation of 100 mL of 0.5 M Sodium fluoride stock solution.

Composition

• 0.5 M Sodium fluoride (NaF) aqueous solution

Required Materials

• Sodium fluoride (NaF) solid (analytical grade)
• Deionized / distilled water
• 100 mL volumetric flask or graduated cylinder
• Analytical balance
• Mixing tool (magnetic stirrer or stirring rod)
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

Follow standard laboratory safety protocols. NaF solutions should be handled with care; ingestion or inhalation of fluoride compounds can be hazardous at high exposure levels.

• Step 1
  Calculate the required mass of NaF solid: For a 0.5 M solution in 0.1 L, Mass = 0.5 mol/L × 0.1 L × 41.99 g/mol ≈ **2.10 g**.
• Step 2
  Accurately weigh ~2.10 g of NaF solid and transfer into a clean container.
• Step 3
  Add ~80 mL deionized water and stir until completely dissolved. Sodium fluoride dissolves rapidly in water.
• Step 4
  Transfer the solution to a 100 mL volumetric flask and adjust to volume with deionized water. Mix thoroughly to ensure homogeneity.

Storage: Label the solution with concentration and date. Store at 4 °C in a tightly capped container for short-term use.

Available Reagents