Sodium chloride Stock Solution

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Stock Solution Preparation Guide – Sodium Chloride (NaCl)

Sodium chloride (NaCl) is a simple inorganic salt widely used in biological, biochemical, and chemical laboratories. It functions as an ionic strength adjuster, a key component in buffer formulations, saline solutions, and cell culture media. The molar mass of sodium chloride is 58.44 g/mol, and this value is used to calculate the mass required for a desired molar concentration.

Chemical Information

Preparation of a 1 M NaCl Solution

A typical procedure for preparing 1 L of a 1 M sodium chloride stock solution is as follows:

1. Calculate the required mass for 1 mol: 58.44 g of NaCl for 1 L of a 1 M solution.
2. Weigh out 58.44 g of sodium chloride on an analytical balance.
3. Place the NaCl into a beaker or volumetric flask and add about 700–800 mL of deionized or distilled water.
4. Stir until the salt is completely dissolved.
5. Transfer the solution to a 1 L volumetric flask (if not already in one) and add deionized water to bring the final volume to exactly 1000 mL.
6. Cap and mix the solution thoroughly; the 1 M NaCl stock is now ready for use.

Notes and Precautions

• Ensure accurate weighing of NaCl to achieve correct molarity.
• Do not add all the solid directly to the full final volume of water; add to part of the water first and then dilute to the final volume.
• Wear appropriate personal protective equipment (lab coat, gloves, goggles) during preparation.

Storage and Usage

• The prepared 1 M NaCl solution can be stored at room temperature or refrigerated if required.
• Common applications include buffer preparation (e.g., PBS), saline solutions, and use as ionic strength modifier in biochemical assays.