Lithium chloride Stock Solution

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Stock Solution Preparation Guide – Lithium Chloride (LiCl)

Lithium chloride (LiCl) is an inorganic salt composed of lithium cations (Li⁺) and chloride anions (Cl⁻) forming white crystalline solids. It is highly soluble in water and hygroscopic, absorbing moisture from air.

LiCl is used in biochemical research (e.g., RNA precipitation), in industrial applications such as moisture control, and as a reagent in chemical synthesis.

Objective

Preparation of 100 mL of 1 M Lithium chloride stock solution.

Composition

• 1 M Lithium chloride (LiCl) aqueous solution

Required Materials

• Lithium chloride (LiCl) or Lithium chloride monohydrate
• Deionized / distilled / Milli-Q water
• 100 mL volumetric flask or measuring cylinder
• Balance for weighing solids
• Magnetic stirrer or manual mixing tool

Procedure

Follow your laboratory’s safety protocols and wear appropriate personal protective equipment.

• Step 1
  Calculate the required mass: for 1 M LiCl, mass = 1 mol/L × 0.1 L × 42.39 g/mol = 4.239 g.
• Step 2
  Accurately weigh ~4.24 g LiCl (anhydrous). If using monohydrate (LiCl·H₂O), adjust proportionally to its molecular weight. Add the solid into ~80 mL of deionized water.
• Step 3
  Stir until completely dissolved, then transfer to a 100 mL volumetric flask and adjust to the final volume with deionized water. Mix thoroughly.

Note: LiCl is hygroscopic and deliquescent; avoid prolonged exposure of the solid to air during weighing.

Sterilization and Storage

For biological applications, sterile-filter the solution through a 0.22 µm membrane. Store at 4 °C for short-term use. For longer storage, aliquot and freeze at −20 °C to minimize degradation and contamination.

Available Reagents