Preparation Glycine Stock Solution

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Stock Solution Preparation Guide – Glycine (NH₂CH₂COOH) Solution

Glycine is the simplest amino acid and is highly soluble in water. It is widely used in biochemical applications including buffer preparation (e.g., for electrophoresis or cell culture reagents) and as a reagent in various solution systems. A common laboratory storage form is a defined‑concentration stock solution (such as 1 M).

Objective

Preparation of a defined concentration stock solution of glycine (e.g., 1 M).

Composition

• Glycine (NH₂CH₂COOH) aqueous solution at specified molarity

Required Materials

• Glycine (solid)
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• pH meter or pH strips (optional if pH adjustment needed)
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Decide the target molarity. For example, to prepare **1 M glycine stock solution**: weigh **75.07 g** of Glycine (MW ≈ 75.07 g/mol) for **1 L** of solution. Adjust proportionally for other volumes (e.g., **7.507 g** for 100 mL).
• Step 2
  Place the weighed Glycine solid into a beaker containing about **80 %** of the target final volume of deionized water. Stir until the solid dissolves completely; Glycine dissolves readily in water.
• Step 3
  Transfer the dissolved solution to a volumetric flask. Rinse the beaker with deionized water and add the rinses to the flask. Add water up to the final desired volume. Mix thoroughly.
• Optional pH Adjustment
  If a specific pH is required (e.g., in buffer systems), adjust the solution pH using small amounts of HCl or NaOH while monitoring with a calibrated pH meter.

Note: Glycine stock solutions are generally stable. If required for sterile applications, filter through a **0.22 µm** membrane into a sterile container. Label the container with concentration and date, and store sealed at room temperature or refrigerated as appropriate.

Available Reagents