Preparation Glucose Stock Solution

The following content was generated by AI and has not been strictly verified; it may contain inaccuracies. All information in the BioCalculator App has been manually curated and carefully validated. You can download the App to view it.

Stock Solution Preparation Guide – Glucose (C₆H₁₂O₆) Solution

Glucose is a carbohydrate widely used in biochemical and cell culture applications as an energy source and to adjust osmolarity. A 1 M glucose stock solution can be prepared by dissolving 18 g of glucose in deionized or distilled water and adjusting the volume to 100 mL. Because glucose is sensitive to thermal degradation, solutions are typically sterilized by filtration rather than autoclaving.

Objective

Preparation of a 1 M glucose stock solution (100 mL).

Composition

• Glucose (C₆H₁₂O₆) aqueous solution at defined molarity

Required Materials

• Glucose powder (C₆H₁₂O₆)
• Deionized or Milli‑Q water
• Analytical balance
• Measuring cylinder
• Conical flask or beaker
• Sterile filter unit (e.g., 0.22 µm)
• Laminar flow hood (for sterile filtration)
• Magnetic stirrer or stirring rod
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  To prepare 100 mL of 1 M glucose solution, accurately weigh **18 g** of glucose and place it into a 250 mL conical flask or beaker.
• Step 2
  Add approximately **80 mL** of deionized or Milli‑Q water to the flask and stir until all glucose dissolves. Glucose dissolves rapidly in water at room temperature.
• Step 3
  Adjust the volume to **100 mL** with deionized or Milli‑Q water. Mix thoroughly.
• Step 4
  Sterilize the solution by filtering through a **0.22 µm** membrane filter unit under sterile conditions (e.g., in a laminar flow hood). Do not autoclave, as high heat can degrade glucose.

Note: Label the stock solution container with concentration and preparation date. Store the sterile glucose solution in the cold room or at appropriate temperature as required by your protocol.

Available Reagents