Dipotassium phosphate Stock Solution

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Stock Solution Preparation Guide – Dipotassium Phosphate (K₂HPO₄)

Dipotassium phosphate (K₂HPO₄) is a water-soluble inorganic salt commonly used in biochemical and molecular biology applications as a buffering component. It dissolves readily in water and provides alkaline phosphate buffering capacity, often used with other phosphate salts to prepare buffers of precise pH.

Chemical Information

Preparation of 1 M Stock Solution

To prepare 1 L of 1 M dipotassium phosphate stock solution:

1. Weigh the appropriate mass of dipotassium phosphate. For anhydrous K₂HPO₄, dissolve 174.18 g; for the trihydrate form (K₂HPO₄·3H₂O), adjust the mass to reflect the correct molarity.
2. Transfer the solid into a suitable beaker or flask and add ~800 mL of deionized or distilled water. Stir until fully dissolved.
3. Once dissolved, transfer to a 1 L graduated cylinder and add deionized water up to the 1000 mL mark. Mix thoroughly.
4. The resulting solution should be clear and colorless. This 1 M stock can be used directly or mixed with other phosphate stocks to prepare buffers of specific pH.

Notes and Precautions

• Dipotassium phosphate is hygroscopic; minimize exposure to air when weighing.
• Add the solid to only part of the final water volume first, then dilute to the final volume to ensure accurate concentration.
• Use appropriate personal protective equipment (gloves, lab coat, goggles).

Storage and Applications

• Store the prepared 1 M stock at room temperature or 4 °C when not in use.
• This stock solution is a key component for preparing phosphate buffer systems at desired pH values when combined with other phosphate salts such as monopotassium phosphate.