Biotin(in PBS) Stock Solution

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Stock Solution Preparation Guide – Biotin in PBS

Biotin (vitamin B7) is a water-soluble vitamin that is only slightly soluble in neutral water but dissolves more readily in warm or alkaline solutions. A 1 mM biotin stock solution can be prepared in phosphate-buffered saline (PBS) for use in biochemical assays and cell culture supplements.

Objective

Preparation of 10 mL of 1 mM Biotin stock solution in PBS.

Composition

• 1 mM Biotin (C₁₀H₁₆N₂O₃S) in PBS

Required Materials

• Biotin (molecular weight 244.31 g/mol)
• Phosphate-buffered saline (PBS)
• 1 N NaOH (optional, to aid dissolution)
• Deionized / distilled water
• 15 mL graduated screw-capped tube
• Vortexer mixer or tube rotator
• Syringe filter (0.22 µm) for sterilization
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Weigh out **2.44 mg** of biotin (for 10 mL of 1 mM stock). Transfer the solid to a 15 mL graduated tube.
• Step 2
  Add **9 mL PBS** and mix (vortex or rotate) to dissolve. If needed, add a few drops of 1 N NaOH to help biotin dissolve; do not dissolve in pure water alone.
• Step 3
  Adjust the solution volume to **10 mL** with deionized water. Mix again.
• Step 4
  Sterilize by filtering through a **0.22 µm syringe filter** into a sterile container under aseptic conditions.

Storage: Aseptically aliquot into small volumes (e.g., 0.5 mL or 1 mL). Store at **–20 °C** until use to maintain stability.

Available Reagents