Ammonium chloride Stock Solution

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Stock Solution Preparation Guide – Ammonium Chloride (NH₄Cl)

Ammonium chloride (NH₄Cl) is a white crystalline inorganic salt with high solubility in water, commonly used in biochemical and analytical laboratory settings. It dissociates into ammonium (NH₄⁺) and chloride (Cl⁻) ions in aqueous solution.

Objective

Preparation of 1 L of 1 M Ammonium Chloride stock solution.

Composition

• 1 M Ammonium chloride (NH₄Cl) in water

Required Materials

• Ammonium chloride (NH₄Cl)
• Deionized / distilled / Milli‑Q water
• Measuring cylinder or volumetric flask (1 L)
• Balance for solid weighing
• Magnetic stirrer or manual mixing device

Procedure

Use appropriate personal protective equipment (lab coat, gloves, goggles) and observe your institutional laboratory safety standards throughout the procedure.

• Step 1
  Accurately weigh 53.49 g of NH₄Cl (Molecular Weight: 53.49 g/mol) for a 1 M solution. Place it into a suitable container.
• Step 2
  Add approximately 800 mL of deionized water and stir until fully dissolved.
• Step 3
  After complete dissolution, transfer to a 1 L volumetric flask (if used) and bring the final volume to 1 L with deionized water. Mix thoroughly.

Note: If a different final volume or molarity is needed, adjust the mass of NH₄Cl proportionally using the molarity equation.
(Molarity = moles/volume).

Sterilization and Storage

Aqueous NH₄Cl stock solutions can be filtered through a 0.22 µm membrane for sterilization if required for biological applications. Solutions are typically stable at 4 °C for several weeks; for long‑term storage, aliquot and freeze at −20 °C to avoid contamination.

Available Reagents