Ammonium acetate Stock Solution

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Stock Solution Preparation Guide – Ammonium Acetate (C₂H₃O₂NH₄) Solution

Ammonium acetate is a white crystalline salt widely used in buffer preparation, protein precipitation, chromatography mobile phases, and analytical applications. It is highly soluble in water. A 1 M stock solution can be prepared by dissolving the appropriate mass of ammonium acetate in water and adjusting the total volume.

Objective

Preparation of a 1 M ammonium acetate stock solution.

Composition

• Ammonium acetate (C₂H₃O₂NH₄) aqueous solution of defined molarity

Required Materials

• Ammonium acetate solid
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Determine the amount of ammonium acetate required. For a 1 M solution, weigh **77.08 g** of ammonium acetate to prepare **1 L** of solution. Adjust proportionally for smaller or larger volumes (e.g., 7.708 g for 100 mL).
• Step 2
  Place the weighed solid into a beaker containing approximately 80 % of the final desired volume of deionized or distilled water. Stir until completely dissolved.
• Step 3
  Transfer the solution to a volumetric flask. Rinse the beaker with small amounts of water to transfer all solute. Add water up to the final volume and mix thoroughly.
• Step 4 (Optional)
  If a sterile solution is required for biological applications, filter through a 0.22 µm membrane filter into a sterile container.

Note: Ammonium acetate decomposes at high temperatures; avoid autoclaving. Label the container with concentration and preparation date. Store the solution in a tightly sealed bottle at 4 °C or at room temperature as appropriate.

Available Reagents