Preparation Solution II (Plasmid isolation)
This guide describes the preparation of Solution II (Plasmid isolation) at a defined molarity for laboratory use.
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Solution II is the alkaline lysis buffer used to lyse bacterial cells during plasmid isolation.
Solution II (Plasmid Isolation) – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Sodium hydroxide | NaOH | 0.2 N | 1310-73-2 |
| SDS | C₁₂H₂₅O₄NaS | 1% (w/v) | 151-21-3 |
Function of Solution II
Solution II is used to lyse bacterial cells by alkaline denaturation of chromosomal DNA and proteins while keeping plasmid DNA intact. SDS helps to solubilize membrane lipids and proteins.
Preparation Tips, Sterilization, and Storage
- The storage period of this solution should preferably not exceed one month.
- SDS readily generates foam. When adding water, allow it to flow slowly down the wall of the container to minimize foaming, and avoid vigorous stirring.
- SDS may form flocculent precipitates at low temperatures; ensure it is fully dissolved before use.
- Sterilization: usually not required; choose as needed.
- Storage: room temperature.
Preview
